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This research outlines the application of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol in animal products. Our assay showed good sensitivity for clenbuterol (0. 4 ng/g or 0. 4 ppb) and low detection limit (0. 09 ng/g or 0. To investigate the metabolic effects of clenbuterol and salbutamol on meat quality of mammals (e. , pork), it is desirable to obtain the metabolic profile of pork tissue by keeping the. A rapid and simple high-performance liquid chromatography-UV method was developed for the separation and quantification of salbutamol, ractopamine, and clenbuterol in pork. A mixture of acetonitrile-formic acid-ammonium acetate was used as the mobile phase to separate three β-agonists on a C18 column with gradient. A low cross-reactivity for other β2-agonist drugs such as salbutamol, terbutaline, and epinephrine led to formatting an ELISA kit considered to have a high specificity for clenbuterol. A survey of Ho Chi Minh City pork market was conducted as part of the validation of our ELISA. ELISA results showed a surprisingly high value of contamination. Furthermore, normal pork samples were clearly differentiated from pork samples with clenbuterol and salbutamol via principal component analysis (PCA). S1 for structure), a kind of β-adrenergic agonist, is not only widely used as a bronchodilator and a tocolytic agent in clinical management, but also illegally used as growth promoters in animal production (Blanca et al It’s disappointing to see that some athletes and individuals still turn to performance-enhancing drugs like Clenbuterol to achieve their goals, despite the many risks involved, us pork clenbuterol.

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